Abstract
Cancer poses significant challenges to global health, necessitating the exploration of innovative therapies. CO2 plasma activated water (PAW) holds promise as a novel therapeutic agent due to its selective cytotoxicity against cancer cells while sparing healthy tissues. This study investigated the in-vitro cytotoxic activity of CO2 PAW against human hepatocarcinoma (HepG2) cells. The cytotoxic effect of CO2 PAW on HepG2 cells was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cells were seeded in 96-well plates, treated with varying volumes of PAW. Morphological changes induced by PAW treatment were assessed using phase-contrast microscopy. The total RNA isolated from HepG2 cells, treated with PAW using the GF-1 total RNA extraction kit, qRT-PCR was performed using SYBR-green master mix with cDNA templates and synthesized primers. Data were analyzed using GraphPad Prism (ver. 9.5, USA, 1992) and presented as mean ± standard error of means. The results revealed dose-dependent cytotoxic effects, with higher concentrations inducing pronounced cell death. Morphological changes in HepG2 cells post-PAW treatment and gene expression analysis showed significant (P<0.05) alterations in Bax and Bcl-2 mRNA levels, indicative of intrinsic apoptotic pathway activation.
Keywords: CO2 Plasma Activated Water, Hepatocellular carcinoma, Cytotoxicity
DOI: https://doi.org/10.55006/biolsciences.2024.4209
Volume: Vol. 4 No. 2 (2024): Volume 4, Issue 2 (June)
Published: Jun 8, 2024
