Studies on analysis of minerals, phytochemicals, proximate composition, and in vitro biological activities of methanolic extracts from the fruits of Cordia dichotoma Forst and Cordia myxa L.

Kainat Zahra; Abdul Samad Khan; Asma Saeed; Hadia Gul

doi:10.55006/biolsciences.2024.4201

Authors

Kainat Zahra Institute of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan
Abdul Samad Khan Institute of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan; Natural Products Research Laboratory, Faculty of Pharmacy, Gomal University, Dera Ismail Khan, Pakistan https://orcid.org/0009-0008-9892-061X
Asma Saeed Institute of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan
Hadia Gul Institute of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan

DOI:

https://doi.org/10.55006/biolsciences.2024.4201

Keywords:

Medicinal Plants, Proximate Analysis, Wild Edible Food Plants , Cordia Myxa, Cordia Dichotoma

Abstract

Plants have been the primary source of sustenance, medicinal remedies, and refuge for humans throughout history. Wild edible fruits, in particular, have played a significant role in shaping people's diets due to their nutritional value. This research aimed to investigate the mineral content, antioxidant properties, chemical composition, and basic characteristics of selected wild edible fruits from Dera Ismail Khan, specifically Cordia myxa and Cordia dichotoma. The chosen wild edible fruits were utilized to determine their inorganic content through spectroscopy, assess their antioxidant potential using the DPPH assay, and analyze their proximate components such as crude fats via the soxhlet extraction method, proteins using the Kjeldahl technique, and ash content through muffle burning. Standard AOAC techniques were employed to ensure accurate measurements. The results of the proximate analysis revealed that C. dichotoma had a carbohydrate content of 88.35%, while C. myxa had 53.79%. Furthermore, the study found that the copper concentrations in C. myxa and C. dichotoma were 525.00 mg/kg and 477.70 mg/kg of dry weight, respectively. Notably, the highest levels of total phenols and their derivatives were detected in C. myxa (33.41 mg GAE/g) and C. dichotoma (19.59 mg GAE/g), with C. myxa exhibiting significant antioxidant activity (IC50 46.75 g/mL). Additionally, both fruits displayed a total flavonoid content of 0.463 mg QE/g. Antibacterial activity was performed by microdilution method of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) whereas, Cordia myxa (L.) showed greatest activity of inhibition against Pseudomonas aeroginosa and Cordia dichotma inhibited Klebsiella pneumonia effectively. Cordia dichotma proved to be the good anti-diabetic while Cordia myxa proved itself for better anti-inflammatory activity in defined laboratory environment, both activities were confirmed by performing enzyme inhibition assays. With high quantities of macronutrients, trace elements, antioxidants, and possible health advantages, these neglected food sources become more important in both conventional and contemporary diets.

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References

Jamshidi-Kia, F., Lorigooini, Z., & Amini-Khoei, H. (2017). Medicinal plants: Past history and future perspective. Journal of herbmed pharmacology, 7(1), 1-7.

Gurib-Fakim, A. (2006). Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular aspects of Medicine, 27(1), 1-93.

Jacob, D. E., Izah, S. C., Nelson, I. U., & Daniel, K. S. (2023). Indigenous Knowledge and Phytochemistry: Deciphering the Healing Power of Herbal Medicine. In Herbal Medicine Phytochemistry: Applications and Trends (pp. 1-53). Cham: Springer International Publishing

Bvenura, C., & Sivakumar, D. (2017). The role of wild fruits and vegetables in delivering a balanced and healthy diet. Food Research International, 99, 15-30.

Shelef, O., Weisberg, P. J., & Provenza, F. D. (2017). The value of native plants and local production in an era of global agriculture. Frontiers in plant science, 8, 2069.

Feyssa, D. H., Njoka, J. T., Asfaw, Z., & Nyangito, M. M. (2011). Seasonal availability and consumption of wild edible plants in semiarid Ethiopia: Implications to food security and climate change adaptation. Journal of Horticulture and Forestry, 3(5), 138-149.

Fouad, A. A., & Rehab, F. M. (2015). Effect of germination time on proximate analysis, bioactive compounds and antioxidant activity of lentil (Lens culinaris Medik.) sprouts. Acta Scientiarum Polonorum Technologia Alimentaria, 14(3), 233-246.

Kim, Y., Singh, M., & Kays, S. E. (2007). Near-infrared spectroscopic analysis of macronutrients and energy in homogenized meals. Food chemistry, 105(3), 1248-1255.

Ahmed, I., Jan, K., Fatma, S., & Dawood, M. A. (2022). Muscle proximate composition of various food fish species and their nutritional significance: A review. Journal of Animal Physiology and Animal Nutrition, 106(3), 690-719.

Augustin, M. A., Riley, M., Stockmann, R., Bennett, L., Kahl, A., Lockett, T., ... & Cobiac, L. (2016). Role of food processing in food and nutrition security. Trends in Food Science & Technology, 56, 115-125.

Ochieno, D. M., Karoney, E. M., Muge, E. K., Nyaboga, E. N., Baraza, D. L., Shibairo, S. I., & Naluyange, V. (2021). Rhizobium-linked nutritional and phytochemical changes under multitrophic functional contexts in sustainable food systems. Frontiers in Sustainable Food Systems, 4, 604396.

Kumar, S., Chauhan, N., Tyagi, B., Yadav, P., Samanta, A. K., & Tyagi, A. K. (2023). Exploring bioactive compounds and antioxidant properties of twenty-six Indian medicinal plant extracts: A correlative analysis for potential therapeutic insights. Food and Humanity, 1, 1670-1679.

Mabry, P. L., Olster, D. H., Morgan, G. D., & Abrams, D. B. (2008). Interdisciplinarity and systems science to improve population health: a view from the NIH Office of Behavioral and Social Sciences Research. American journal of preventive medicine, 35(2), S211-S224.

Soetan, K. O., Olaiya, C. O., & Oyewole, O. E. (2010). The importance of mineral elements for humans, domestic animals and plants: A review. African journal of food science, 4(5), 200-222.

Son S., Lewis B. A., (2002), J. Agric. Food Chem., 50, 468—472 (2002).

Li, N., Zhang, D. S., Liu, H. S., Yin, C. S., Li, X. X., Liang, W. Q., ... & Zhang, D. B. (2006). The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development. The Plant Cell, 18(11), 2999-3014.

Kaur, G. J., & Arora, D. S. (2009). Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC complementary and alternative medicine, 9, 1-10.

Gill, B. D., Indyk, H. E., Blake, C. J., Konings, E. J., Jacobs, W. A., & Sullivan, D. M. (2015). Evaluation protocol for review of method validation data by the AOAC stakeholder panel on infant formula and adult nutritionals expert review Panel. Journal of AOAC International, 98(1), 112-115.

Indrayan, A. K., Sharma, S., Durgapal, D., Kumar, N., & Kumar, M. (2005). Determination of nutritive value and analysis of mineral elements for some medicinally valued plants from Uttaranchal. Current science, 1252-1255.

Rangana, S. (1979). Manual of analysis of fruit and vegetable products. Tata McGraw-Hill.

Madhubala, M., & Santhi, G. (2019). Phytochemical and GC-MS analysis on leaves of selected medicinal plants in Boraginaceae family Cordia dichotoma L. Pramana Res. J, 9, 2249-2276.

Singh, A., Nagpoore, N. K., Singh, B. N., & Thakur, M. (2024). Comprehensive analysis of micro and macro-minerals, phytochemicals and proximate composition of Cordia dichotoma G. forst fruits. Journal of Food Science and Technology, 1-10.

Abdel-Aleem, E. R., Attia, E. Z., Farag, F. F., Samy, M. N., & Desoukey, S. Y. (2019). Total phenolic and flavonoid contents and antioxidant, anti-inflammatory, analgesic, antipyretic and antidiabetic activities of Cordia myxa L. leaves. Clinical Phytoscience, 5, 1-9.

Gupta, R., & Kaur, J. (2015). Evaluation of analgesic, antipyretic and anti-inflammatory activity on Cordia dichotoma G. Forst. Leaf. Pharmacognosy Research, 7(1), 126.

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical microbiology reviews, 12(4), 564-582.

Arunachalam, K., & Parimelazhagan, T. (2014). Evaluation of phenolic content, antioxidant activity, and nutritional composition of Cordia evolutior (Clarke) Gamble. International journal of food properties, 17(1), 226-238.