In silico identification of hemicellulase producing microorganisms found in animal manure

Ariha Afsheen; Sabeen Sabri; Fareeha Afsheen; Rida Javed; Rabia Akhtar; Mukarram Sharif

doi:10.55006/biolsciences.2023.3403

Authors

Ariha Afsheen Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara 56130, Pakistan https://orcid.org/0009-0002-5775-528X
Sabeen Sabri Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara 56130, Pakistan; Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, University of the Punjab, Lahore 54590, Pakistan
Fareeha Afsheen Department of Bioinformatics, Faculty of Life Sciences, University of Okara, Okara 56130, Pakistan https://orcid.org/0009-0005-5640-8798
Rida Javed Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara 56130, Pakistan https://orcid.org/0009-0000-2303-3866
Rabia Akhtar Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara 56130, Pakistan
Mukarram Sharif Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara 56130, Pakistan https://orcid.org/0009-0009-4879-4149

DOI:

https://doi.org/10.55006/biolsciences.2023.3403

Keywords:

Xylanases, Hemicellulases, Xylan, Fungi, Bacteria, Lignocellulosic biomass, Geobacillus strearothermophilus, Myceliophthora thermophila, Claviceps purpurea, Bioinformatics, Phylogenetic tree

Abstract

To speed up biological processes in all living things, nature has produced enzymes. In industries like leather, textile, food, paper, detergents, medicines, and feed, a variety of enzymes act as industrial biocatalysts, and their usage in commercial chemical reactions is expected to improve significantly. This study aims to find out the high activity of xylanase-producing microorganisms to reduce the cost of raw materials that are used in several commercial industries like paper pulp bleaching, bioconversion of lignocellulosic biomass into biofuels, etc. This study was conducted to characterize and identify the most highly efficient hemicellulose degrading enzymes from bacterial and fungal species by using bioinformatics methodologies. in this study, 27 xylanase-producing bacterial sequences and 13 xylanase-producing fungal sequences were retrieved from the NCBI data bank (http://www.ncbi.org). The predicted microorganisms were further analyzed by retrieving protein sequences from uniprot. This study concluded that Geobacillus strearothermophilus and Claviceps purpurea, exhibit a high level of specific enzyme activity as compared to target bacterial sp. Myceliophthora thermophila and fungal sp. Aspergillus nigar respectively at the favorable temperature of 60◦C with optimal neutral pH. The identified enzymes will be helpful in the reduction of cost and energy required in the conversion of biomass or in various other industrial sectors for the processing of raw material.

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