Ameliorative Effects of Curcumin on Ciprofloxacin-Induced Testicular Toxicity: A Study on Reproductive Hormones, Oxidative Stress, and Histological Changes in Adult Male Mice

Edache Daniel Abah; Daniel David Eriba; Paul Oda Omirida; Monday Francis Ukase; Fabian Deungwan Maikyo

doi:10.55006/biolsciences.2024.4404

Authors

Edache Daniel Abah Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Benue State University, Makurdi https://orcid.org/0009-0005-3690-0902
Daniel David Eriba Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Benue State University, Makurdi https://orcid.org/0009-0009-1476-6342
Paul Oda Omirida Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Benue State University, Makurdi https://orcid.org/0009-0005-5760-9866
Monday Francis Ukase Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Benue State University, Makurdi https://orcid.org/0009-0006-1046-0694
Fabian Deungwan Maikyo Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Benue State University, Makurdi https://orcid.org/0009-0007-9317-805X

DOI:

https://doi.org/10.55006/biolsciences.2024.4404

Keywords:

Curcumin, Ciprofloxacin, Testicular toxicity, Oxidative stress, Reproductive hormones, Histology

Abstract

Ciprofloxacin, a widely used fluoroquinolone antibiotic, has been associated with testicular toxicity, characterized by oxidative stress and hormonal imbalances that impact male reproductive health. Curcumin, a natural antioxidant and anti-inflammatory agent, has shown potential in mitigating these effects. This study aimed to evaluate the ameliorative effects of curcumin on ciprofloxacin-induced testicular toxicity in adult male mice, focusing on reproductive hormones, oxidative stress, and histological changes. Thirty mice were divided into six groups, receiving varying doses of ciprofloxacin and curcumin over 30 days. Hormonal assays for testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were performed, alongside assessments of oxidative stress markers like malondialdehyde (MDA) and histopathological analyses of testicular tissue. Results showed a significant reduction in FSH and testosterone levels in ciprofloxacin-treated mice, with curcumin ameliorating these effects in co-treatment groups. MDA levels, an indicator of oxidative stress, were elevated in ciprofloxacin-only groups but significantly reduced in curcumin-treated groups. Histological examination revealed that curcumin protected against ciprofloxacin-induced testicular damage. In conclusion, curcumin demonstrated significant protective effects against ciprofloxacin-induced testicular toxicity by normalizing reproductive hormone levels, reducing oxidative stress, and preserving testicular architecture. These findings suggest curcumin as a potential therapeutic agent for preventing fluoroquinolone-related reproductive toxicity.

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