Antibacterial, Acute Toxicity and Histological Study of Dodonaea viscosa, Eucalyptus camaldulencis and Senna siamae: Ethnobotanical Plants for Typhoid Fever in Kano State, Nigeria

Sakina Sani Buhari

doi:10.55006/biolsciences.2026.6101

Authors

Sakina Sani Buhari Department of Biology, Aliko Dangote University of Science and Technology, Wudil, Kano State, Nigeria

DOI:

https://doi.org/10.55006/biolsciences.2026.6101

Keywords:

Antibacterial, Dodonaea viscosa, Eucalyptus camaldulensis, Senna siamae, Acute toxicity, Histology

Abstract

This study evaluated the antibacterial activity, acute toxicity, and histological effects of Eucalyptus camaldulensis, Dodonaea viscosa, and Senna siamae, three ethnobotanical plants traditionally used to treat typhoid fever in Kano State, Nigeria. Antibacterial activity against Salmonella typhi was assessed using the agar well diffusion method, followed by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Acute toxicity was evaluated using a single oral limit dose of 5000 mg/kg in mice, and histological assessment of the liver, kidney, and heart was performed using hematoxylin-eosin staining. At 100 mg/ml, E. camaldulensis exhibited the largest inhibition zone (20.3 ± 2.52 mm), followed by S. siamae (15.0 ± 0.00 mm) and D. viscosa (13.0 ± 0.00 mm) which showed a statistically significant (p < 0.05) with the control. MIC and MBC values confirmed that E. camaldulensis had the highest antibacterial potency (MIC 6.25 mg/ml; MBC 12.5 mg/ml), whereas D. viscosa and S. siamae showed MIC 12.5 mg/ml and MBC 25 mg/ml. Acute toxicity assessment revealed no mortality at 5000 mg/kg for all the extracts (LD₅₀ > 5000 mg/kg). Histological evaluation showed normal heart tissues in all the tested groups, normal liver and kidney tissues in E. camaldulensis and S. siamae-treated mice, but slight tubular necrosis in the kidney and minimal hepatic necrosis in the liver of D. viscosa-treated mice. Lesions were graded as minimal and observed in 1 out of 3 mice per group.

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References

1. Akinyemi KO, Oyefolu AOB, Mutiu WB, Iwalokun BA, Ayeni ES, Ajose SO, et al. Typhoid fever: tracking the trend in Nigeria. Am J Trop Med Hyg. 2018;99(3):41–47.

2. Lauteri C, Maggio F, Serio A, Festino AR, Paparella A, Vergara A. Overcoming multidrug resistance in Salmonella spp. isolates obtained from the swine food chain by using essential oils: an in vitro study. Front Microbiol. 2022;12:808286.

3. Buhari SS, Ajingi YA, Haruna M, Yakasai MA, Muhammed U, Ahmad B. Kano State commercial botanical gardens: ethnobotanical survey of medicinal plants grown therein for the treatment of various human ailments. FUDMA J Sci. 2025;9(2):248–255.

4. Taj HM, Zubaira F, Riaz N, Taj S, Amjad N, Iqbal A, et al. Eucalyptus camaldulensis for combating multidrug-resistant bacterial strains. Braz J Biol. 2024;84:e282826.

5. Ayepola OO, Adeniyi BA. The antibacterial activity of leaf extracts of Eucalyptus camaldulensis (Myrtaceae). J Appl Sci Res. 2008;4(11):1410–1413

6. Adamu U, Yusha’u M. In vitro antibacterial activity and toxicity study of Eucalyptus camaldulensis leaf extract against clinical isolates of Salmonella spp. Dutse J Pure Appl Sci. 2018;4(2):213–220

7. Doughari JH, Manzara S. In vitro antibacterial activity of crude leaf extracts of Eucalyptus camaldulensis against some bacteria causing nosocomial infection. Afr J Microbiol Res. 2008;2(11):37–41

8. Sani A, Abdulhamid F, Bello. Eucalyptus camaldulensis: phytochemical composition of ethanolic and aqueous extracts of the leaves, stem bark, root, fruits and seeds. J Sci Innov. 2014;3(5):523–526

9. Waweru AW, Osoro EK, Omolo JO. A review on the phytochemistry and pharmacological activities of some species from genus Dodonaea (Sapindaceae family). J Phytopharmacol. 2024;13(1):70–76

10. Shafek RE, Shafik NH, Michael HN, El-Hagrassi AM, Osman AF. Phytochemical studies and biological activity of Dodonaea viscosa flowers extract. J Chem Pharm Res. 2015;7(5):109–116

11. Bangajavalli S. Pharmacognostical and phytochemical studies of Dodonaea viscosa (L.) Jacq. (Sapindaceae). Int Res J Plant Sci. 2023;14(2):1–10

12. Kumar MS, Selvakumar S, Rao MRK, Anbuselvi S. Phytochemical analysis of Dodonaea viscosa. Asian J Plant Sci Res. 2013;3(1):43–46

13. Nas FS, Ali M, Yahaya A, Zainab M. Antibacterial activity of Senna siamae. J Pharm Biol Sci. 2018;13(2):45–52

14. Igbinosa OO, Igbinosa EO. Antimicrobial activity and phytochemical screening of Cassia fistula and Senna siamae leaf extracts. Afr J Biotechnol. 2009;8(12):2450–2454

15. Sani SB, Surayya LI, Binta MS, Namadina MM, Aisha MG, Mardiyya AY, et al. Phytochemical analysis and antioxidant potential of ethanol extracts of Anogeissus leiocarpus and Acacia nilotica. Biol Sci (Int J Med Pharm Life Sci). 2024;4(1):525–534.

16. Al-Azawi AH. Phytochemical, antibacterial and antioxidant activities of Dodonaea viscosa Jacq. extracts cultivated in Iraq. Iraqi J Biotechnol. 2017;16(4):37–46

17. Azza HHD, Shayoub ME, Shayoub SME. Acute toxicity studies of ethanolic extract of Eucalyptus camaldulensis Dehnh leaves. J Netw Commun Emerg Technol. 2015;2(2):1–6

18. Ejike UDI, Anunobi OO. Antioxidant, dermal and acute toxicological effects of Eucalyptus camaldulensis essential oil on male Wistar rats. J Phytomed Ther. 2022;21(2):891–905.

19. Khurram M, Khan MA, Hameed A, Abbas N, Qayum A, Inayat H. Antibacterial activities of Dodonaea viscosa using contact bioautography technique. Molecules. 2009;14:1332–1341.

20. Sani SB, Aliyu BS. Determination of antibacterial activity of five medicinal plants traditionally used for the treatment of diabetic foot infection in Kano State, Nigeria. Bayero J Pure Appl Sci. 2022;13(1):305–312.

21. Khalil NM, Sperotto JS, Manfron MP. Anti-inflammatory activity and acute toxicity of Dodonaea viscosa. Fitoterapia. 2006;77(6):478–480.

22. Jour TY, Wiam IM, Jacks T, Zongoma YA. Acute toxicity and phytochemical studies of Cassia siamea extract in rats. Pak J Biol Sci. 2005;10:586–588

23. Khan MF, Al Qahtani AS, Almarfadi OM, Ullah R, Nasr FA, Noman OM, et al. Reproductive toxicity associated with Dodonaea viscosa, a folk medicinal plant in Saudi Arabia. Evid Based Complement Alternat Med. 2021;2021:6689110.