Myricetin loaded nanoemulgel: in vitro characterization and anti- inflammatory efficacy assessment in Sprague Dawley rats

Nazneen Sultana
Usama Ahmad
Juber Akhtar


Myricetin, a commonly found natural flavonoid, exhibit a wide range of potential therapeutic activity including antidiabetic, anticarcinogenic, neuroprotective, and antiinflammatory activity. It belongs to BCS class II and has poor aqueous solubility, which limits its therapeutic application in the treatment therapy. This study was aimed to design and characterize the nanoemulgel formulation of Myricetin for topical delivery with the possibility of improved solubility, skin penetration and antiinflammatory activities. In this work Myricetin loaded nanoemulgel was prepared by water titration method and evaluated for particle size, zeta potential, pH, spreadability, rheology, drug content, skin permeation, skin irritation studies. In-vivo anti-inflammatory study of optimized Myricetin nanoemulgel was compared with marketed preparation (Voveran® emulgel®). The prepared Myricetin nanoemulgel containing Carbopol 934P (2%) as a gelling agent, Tween 20 (20%) as a surfactant, Ethanol (20%) as a co-surfactant and Sefsol 218 (20%) as an oil. The optimized Myricetin nanoemulgel was highly stable and it showed no sign of edema, skin irritation. It also showed significant anti-inflammatory activity. Transdermal permeation study demonstrated higher penetration of Myricetin from nanoemulgel compared to the marketed emulgel (Voveran® emulgel®). The Myricetin nanoemulgel showed promising results and can be further considered as a potent natural alternative to the synthetic drug for the treatment of inflammation.

DOI: 10.55006/biolsciences.2024.4202
Published: 08-06-2024

How to Cite
Sultana, N., Usama Ahmad, Badruddeen, & Akhtar, J. (2024). Myricetin loaded nanoemulgel: in vitro characterization and anti- inflammatory efficacy assessment in Sprague Dawley rats. Biological Sciences, 4(2), 585–597.


Kim HH, Kim DH, Kim MH, et al. Flavonoid constituents in the leaves of Myrica rubra sieb. et zucc. with anti-inflammatory activity. Arch Pharm Res. 2013;36(12):1533-1540.

Semwal DK, Semwal RB, Combrinck S, Viljoen A. Myricetin: A dietary molecule with diverse biological activities. Nutrients. 2016;8(2):1–31.

Wang L, Wu H, Yang F, Dong W. The Protective Effects of Myricetin against Cardiovascular Disease. J Nutr Sci Vitaminol. 2019;65(6):470-476.

Dang Y, Lin G, Xie Y, Duan J, Ma P, Li G, et al. Quantitative determination of myricetin in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its absolute bioavailability. Drug Res (Stuttg). 2013;64(10):516–22.

Lu L, Qian D, Guo J, Qian Y, Xu B, Sha M, et al. Abelmoschi Corolla non-flavonoid components altered the pharmacokinetic profile of its flavonoids in rat. J Ethnopharmacol. 2013;148(3):804–11.

Karashima M, Kimoto K, Yamamoto K, Kojima T, Ikeda Y. A novel solubilization technique for poorly soluble drugs through the integration of nanocrystal and cocrystal technologies. Eur J Pharm Biopharm. 2016;107:142–50.

Yousaf AM, Mustapha O, Kim DW, Kim DS, Kim KS, Jin SG, et al. Novel electrosprayed nanospherules for enhanced aqueous solubility and oral bioavailability of poorly watersoluble fenofibrate. Int J Nanomedicine. 2016;11:213–21.

Eid AM. Preparation, Characterization and Anti-Inflammatory Activity of Swietenia macrophylla Nanoemulgel. J Nanomed Nanotechnol. 2014;05(02).

Chellapa P, Mohamed AT, Keleb EI, Elmahgoubi A, Eid AM, Issa YS, et al. Nanoemulsion and Nanoemulgel as a Topical Formulation. IOSR J Pharm. 2015;5(10):43–7.

Sultana N, Chauhan D, Yadav PK, et al. Enhanced Oral Bioavailability of Isoformononetin Through Nanoemulsion: Development, Optimization, and Characterization. J Pharm Innov. 2024;19(8)

Sultana N, Akhtar J, Badruddeen, Irfan Khan M, Ahmad U, Arif M, et al. Nanoemulgel: For Promising Topical and Systemic Delivery. Drug Development Life Cycle. IntechOpen; 2022.

Preeti, Sharda S, Rohit M, Saurabh B, Ahmed AH, Chanchal R, Renu S, Suresh K, Geeta, RS, Nanoemulsion: An Emerging Novel Technology for Improving the Bioavailability of Drugs. Scientifica. 2023; 2023(25)

Ali A, Ansari VA, Ahmad U, Akhtar J, Jahan A. Nanoemulsion: An Advanced Vehicle for Efficient Drug Delivery. Drug Res (Stuttg). 2017;67(11):617–31.

Jeengar MK, Rompicharla SVK, Shrivastava S, Chella N, Shastri NR, Naidu VGM, et al. Emu oil based nano-emulgel for topical delivery of curcumin. Int J Pharm [Internet]. 2016;506(1–2):222–36. Available from:

Sinica DP, Begur M, Pai VK, Gowda D V, Srivastava A, Raghundan H V, et al. Pelagia Research Library Enhanced permeability of Cyclosporine from a transdermally applied nanoemulgel. 2015;6(2):69–79.

Hardenia A, Road K. Emulgel: an emergent tool in topical drug delivery Anu Hardenia * , Sonali Jayronia and Sanjay Jain Smriti College of Pharmaceutical Education, 4/1 Pipliya Kumar Kakad, Maya Khedi Road, Indore 452010, (MP), India. 2014;5(5):1653–60.

Aithal GC, Nayak UY, Mehta C, Narayan R, Gopalkrishna P, Pandiyan S, et al. Localized in situ nanoemulgel drug delivery system of quercetin for periodontitis: Development and computational simulations. Molecules. 2018;23(6).

Mulia K, Ramadhan RMA, Krisanti EA. Formulation and characterization of nanoemulgel mangosteen extract in virgin coconut oil for topical formulation. MATEC Web Conf. 2018;156:01013.

Bhattacharya S, Prajapati BG. Formulation and optimization of celecoxib nanoemulgel. 2017;10(8).

Setya S, Razdan BK, Talegaonkar S, Tariq M, Madaan T. Appraisal of Transdermal Waterin-Oil Nanoemulgel of Selegiline HCl for the Effective Management of Parkinson’s Disease: Pharmacodynamic, Pharmacokinetic, and Biochemical Investigations. AAPS PharmSciTech. 2017;19(2):573–89.

Dasgupta S, Dey S, Choudhury S, Mazumder B. Topical delivery of aceclofenac as nanoemulsion comprising excipients having optimum emulsification capabilities: preparation, characterization and in vivo evaluation. Expert Opin Drug Deliv. 2013;10(4):411-420.

Radhika PR, Guruprasad S. Nanoemulsion based emulgel formulation of lipophilic drug for topical delivery. Int J PharmTech Res. 2016;9(6):210–23.

Srivastava M, Kohli K, Ali M. Formulation development of novel in situ nanoemulgel (NEG) of ketoprofen for the treatment of periodontitis. Drug Deliv. 2016;23(1):154–66.

Zhao Y, Peng F, Ke Y. Design and characterization of oil-in-water nanoemulsion for enhanced oil recovery stabilized by amphiphilic copolymer, nonionic surfactant, and LAPONITE® RD. RSC Adv. 2021;11(4):1952-1959. Published 2021 Jan 7.

Aggarwal G, Dhawan B, Harikumar S. Enhanced transdermal permeability of piroxicam through novel nanoemulgel formulation. Int J Pharm Investig. 2014;4(2):65.

Khurana S, Jain NK, Bedi PMS. Nanoemulsion based gel for transdermal delivery of meloxicam: Physico-chemical, mechanistic investigation. Life Sci [Internet]. 2013;92(6–

:383–92. Available from:

Arora R, Aggarwal G, Harikumar SL, Kaur K. Nanoemulsion Based Hydrogel for Enhanced Transdermal Delivery of Ketoprofen. Adv Pharm. 2014;2014:1–12.

Duarah S, Durai RD, Narayanan VHB. Nanoparticle-in-gel system for delivery of vitamin C for topical application. Drug Deliv Transl Res. 2017;7(5):750–60.

Dhawan B, Aggarwal G, Harikumar S. Enhanced transdermal permeability of piroxicam through novel nanoemulgel formulation. Int J Pharm Investig. 2014;4(2):65-76.

Gurpreet K, Singh SJ. Reviewof nanoemulsion formulation and characterization techniques. Indian Journal of Pharmaceutical Sciences.2018;80(5):781-789.

Baboota S, Shakeel F, Ahuja A, Ali J, Shafiq S. Design, development and evaluation of novel nanoemulsion formulations for transdermal potential of celecoxib. Acta Pharm. 2007;57(3):315–32.

Shao Y. Research Article. SciFed Food Dairy Technol J. 2017;1(1):132–5.

Choudhury H, Gorain B, Pandey M, Chatterjee LA, Sengupta P, Das A, et al. Recent Update on Nanoemulgel as Topical Drug Delivery System. J Pharm Sci [Internet].

;106(7):1736–51. Available from:

Miller MA, Zachary JF. Mechanisms and Morphology of Cellular Injury, Adaptation, and Death. Pathologic Basis of Veterinary Disease. 2017;2-43.