Comparative Antimicrobial Properties of a Consortium of Nauclea latifolia Sm. and Ocimum gratissium L. Extracts with Their CuO
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Abstract
Over the years, several microorganisms have developed antibiotic resistance, rendering them impotent and otherwise useless. This research work is intended at exemplifying the consortium of crude extracts of Nauclea latifolia and Ocimum gratissium for antimicrobial potentials. Nanoparticles made from extracts were equated to the activities. Plant nanoparticles were created by combining powdered plant materials with copper oxide and suspending them in ethanol and distilled water, which were then screened to achieve crude extracts (CuO). The presence of bioactive machinery tartan in crude extracts. Agar well diffusion was betrothed to test antibacterial activity while the poisoned plate method was utilized to define antifungal potentials. Flavonoids, alkaloids, steroids, saponins, terpenoids, and tannins were discovered in plant extracts. At a dosage of 40 mg/mL, the aqueous crude extract outperformed the ethanolic extract, with the best inhibition zone (30) against Bacillus megaterium. The antimicrobial properties of the aqueous nanoparticle extract were stronger than those of the crude equivalent, antibacterial and antifungal inhibition zones were 36 mm at 40 mg/mL and 32 mm at 60 mg/mL respectively. According to the findings, aqueous extracts have stronger antibacterial activity than ethanolic extracts and nanoparticle created utilizing a syndicate of two plants has the potential to improve antimicrobial properties.
References
Urits, I., Schwartz, R. H., Orhurhu, V., Maganty, N. V., Reilly, B. T., Patel, P. M., Wie, C., Kaye, A. D., Mancuso, K. F., Kaye, A. J., Viswanath, O. (2021). A Comprehensive Review of Alternative Therapies for the Management of Chronic Pain Patients: Acupuncture, Tai Chi, Osteopathic Manipulative Medicine, and Chiropractic Care. Advances in Therapy, 38(1):76-89.
Iheagwam, F. N., Israel, E. N., Kayode, K. O., DeCampos, C., Ogunlana, O. O., Chinedu, S. N. (2020). Nauclea latifolia Sm. Leaf Extracts Extenuates Free Radicals, Inflammation, and Diabetes-Linked Enzymes. Oxidative Medicine and Cellular Longevity, 2020:5612486.
Oke, E. O., Okolo, B. I., Adeyi, O., Agbede, O. O. , Nnaji, P, C., Adeyi, J. A. , Osoh, K. A., Ude. C. J. (2020). Black-box modeling, bi-objective optimization, and ASPEN batch simulation of phenolic compound extraction from Nauclea latifolia root. Heliyon, 7(1): e05856.
Ugbogu, O. C., Emmanuel, O., Agi, G. O., Ibe, C., Ekweogu, C. N., Ude, V. C., Uche, M. E., Nnanna, R. O., Ugbogu, E. A. (2021). A review of the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.). Heliyon, 7(11): e08404.
Zhan, Y., An, X., Wang, S., Sun, M., Zhou, H. (2020). Basil polysaccharides: A review on extraction, bioactivities and pharmacological applications. Bioorganic and Medicinal Chemistry, 28(1):115179.
Balaji, R., Ravichandiran, K., Tanuja and Parani, M. (2021). The complete chloroplast genome of Ocimum gratissimum from India - a medicinal plant in the Lamiaceae. Mitochondrial DNA Part B Resources, 6(3):948-950.
Kozsup, M., Zhou, X., Farkas, E., Cs Bényei, A., Bonnet, S., Patonay, T., Kónya, K., Buglyó, P. (2021). Synthesis, characterization and cytotoxicity studies of Co(III)-flavonolato complexes. Journal of Inorganic Biochemistry, 217:111382.
Eilers, A., Witt, S and Walter, J. (2020). Aptamer-Modified Nanoparticles in Medical Applications. Advances in Biochemical Engineering/ Biotechnology. 174:161-193.
Chen, T., Qi, Y., Wang, L and Li, C. (2021). First Report of Leaf Spot Disease on Fagopyrum esculentum caused by Bipolaris zeae in China. Plant Diseases. doi: 10.1094/PDIS-01-21-0020-PDN. Online ahead of print.
Pirtarighat, S., Ghannadnia, M and Baghshahi, S. (2019). Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. Journal of Nanostructure in Chemistry, 9(1):1-9.
Ahmed, R. N., Sani, A., Ajiboye, A. E., Gambari-Ambali, R. O., Ezekiel, Ku. (2013). Sensitivity of three gastrointestinal organisms to aqueous extract of leaf of Ocimum gratissimum. Nigeria Journal of Pure and Applied Sciences, 26:2460- 2469.
Ahmed, R. N., Sani, A., Igunnugbemi, O. O. (2009). Antifungal profiles of extracts of Vitellaria paradoxa (Shea-Butter) bark. Ethnobotanical leaflets, 13:679-688.
Karim, R.(2020). Identification of phytochemicals from North African plants for treating Alzheimer's diseases and of their molecular targets by in Silico network pharmacology approach. Journal of Traditional and Complementary Medicine, 11(3):268-278.
Soliman, E. S., Hamad, R.T and Abdallah, M. S. (2021). Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers. Veterinary World, 14(2):523-536.
Ștefănescu, R., Tero-Vescan, A., Negroiu, A., Aurică, E and Camil-Eugen, Vari, C. E. (2020). A Comprehensive Review of the Phytochemical, Pharmacological, and Toxicological Properties of Tribulus terrestris L. Biomolecules, 10(5):752.
Tomee, J. F and Kauffman, H. F. (2000). Putative virulence factors of Aspergillus fumigatus. Clinical and Experimental Allergy. 30(4):476-484.
Mabhiza, D., Chitemerere, T and Mukanganyama, S. (2016). Antibacterial Properties of Alkaloid Extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. International Journal of Medicinal Chemistry; 2016:6304163.
Bamigboye, Oluwaseyi Mercy., Ahmed, R. N. (2019). Comparative Antimicrobial Activities of a Consortium of Vernonia amygdalinaand Amaranthus hybridus Extracts With Their CuO Nanoparticle Complexes. International Journal of Medicinal Review, 6(1):31–34.
Gazi, U , Rosas, M., Singh, S., Heinsbroek, S., Haq I., Johnson, S., Brown, B. G., Williams, D. L., Taylor, P. R., Martinez-Pomares, L. (2011). Fungal recognition enhances mannose receptor shedding through dectin-1 engagement. Journal of Biological Chemistry, 286(10):7822-7829.
Prabhu, S and Poulose, E. K. (2012). Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letter. 2(1):32.
Sabnis, A., Katheryn, L. H ., Klöckner, A., Becce, M., Lindsay, Evans, L. E., Furniss, R. C., Mavridou, D. A., Murphy, R., Stevens, M. M., Davies, J.C., Larrouy-Maumus, J. G., Clarke, T. B and A. M. (2021). Colistin kills bacteria by targeting lipopolysaccharide in the cytoplasmic membrane. Elife,10: e65836.
Ellis, B. M, Babele, P. K., May, J. C., Johnson, C. H., Pfleger, B. F., Young, J. D and McLean, J. A. (2021). Accelerating strain phenotyping with desorption electrospray ionization-imaging mass spectrometry and untargeted analysis of intact microbial colonies. Proceeding of the National Academy of Sciences USA, 118(49): e2109633118.