Sesamum indicum (Beniseed) Oil as a Potential Therapeutic Agent for Testicular Toxicity in Stressed and Sleep–Deprived Male Wistar Rats

Abstract

The testicles play a crucial role in male reproductive health by producing sperm and synthesizing androgens, primarily testosterone. This study investigates the potential of Sesamum indicum (Beniseed) oil as a therapeutic agent against testicular toxicity induced by chronic psychological stress and sleep deprivation in male Wistar rats. Twenty-five rats were divided into five groups and subjected to various stress and sleep deprivation protocols over 14 days, with groups receiving different doses of Beniseed oil. Reproductive hormone levels, sperm parameters, and testicular histology were evaluated. Results indicated that both short-term and long-term stress and sleep deprivation negatively affected follicle-stimulating hormone (FSH) and testosterone levels, with Beniseed oil showing limited protective effects. Notably, low doses of Beniseed oil improved sperm count in some groups, while high doses exacerbated hormonal declines and did not mitigate histological damage. Histological analysis revealed disrupted seminiferous tubules and cellular debris across treatment groups, indicating persistent testicular damage despite Beniseed oil administration. This study highlights the limited efficacy of Beniseed oil in reversing testicular damage caused by stress and sleep deprivation, suggesting that while it may offer some protective effects at low doses, higher doses could be counterproductive.

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